Article Type
Article
Abstract
Food borne pathogens are major impediment of the public health and economic development, mostly Enterobacteriaceae is inherent foodborne pathogens of which E. herbicola have recently emerged significant in ready eat food. The aim of this study was to determine quantitatively of viable bacterial isolates and molecular diagnosis of Erwinia herbicola by SYBR Green real-time PCR in nuts. The bacterial species isolates of nuts are Coagulase + Staphylococcus, E. coli, Enterobacter cloacae and Erwinia herbicola. The highest rate of Coagulase + Staphylococcus species of two hazelnuts and two cashew samples and Erwinia herbicola of one almond sample, theses bacterial isolates from nuts were present between microbiological unsatisfactory and marginal levels, however in present study selected E. herbicola to molecular identification by used SYBR Green qPCR assay to amplify the rep gene. The specificity of assay was represented molecular quantitation correlation was between ct values and enumeration on aerobic plate agar count of bacteria in nuts, the results showed that assay is
Keywords
Enterobacteriaceae, SYBR Green qPCR, Staphylococcus
Recommended Citation
Al-Miyah, Suaad Abid Fazaa
(2025)
"Molecular detection of Erwinia herbicola isolated from Nuts,"
Muthanna Medical Journal: Vol. 4:
Iss.
2, Article 15.
Available at:
https://muthmj.researchcommons.org/journal/vol4/iss2/15
Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.